General procedures and the use of spectrophotometer UV-Visible

(i) Sample dissolved in the solvent
(ii) The sample included in kuvet
(iii) In the closed state, set T = 0% (in some instruments, it is called 0% T. Dark current control)
(iv) In an open, set T = 100% (A = 0). Use a cell filled with pure solvent
(v) Enter the sample and measuring% T (or A)