3.29.2010

Instrumentation on the UV-Vis spectrophotometer (visible light)

Lights:
(a) Tungsten Light, stable, inexpensive, 350-1000nm
(b) tungsten halogen lamp (quartz-iodine lamp) with a tungsten light but has a better output in the region 300-400nm
(c) Deuterium Arc Lamps, expensive, short tenure, 190-400nm

Dispersion System
(a) Filter
Only used on cheap tape colourimeter ≈ 25-50 nm dalm not commonly used modern instruments
(b) Prism
quartz prism has a weak dispersion characteristics in the area seem sianr (380-780 nm) varies according dispersion wavelength is more expensive than the grating
(c) Difractions Gratings
Dispersion in cash gelombnag length greater than that usually used.

Kuvet
(a) Glass
Commonly used (in the 340-1000 nm) usually has a length of 1 cm (or 0.1, 0.2, 0.5, 2 or 4 cm)
(b) Quartz
expensive, range (190-1000nm) (c) Automatic Cell (flow through cells)
(d) Matched cells
(e) polystyrene range (340-1000nm) throw away type
(f) Micro cells

Detector
(a) Barrier-layer cell (photo cell or photo cell Voltaïc)
(b) photo tube, more sensitive than the photo cell, requires a stable power supply and amplifier
(c) photo multipliers, very sensitive, fast response is used in double-beam instrument internal reinforcement

Reader system
(a) Null balance, using a null-balance principle Potentiometer, not comfortable, many replaced by a direct reading and reading of digital
(b) Direct readers,% T, A or C is read directly from the scale
(c) digital readings, changing analog to digital signals and display the exhibit number Light emitting diode (LED) as A,% T or C. With the meter reading as a picture, it's easier to read transmitannya scale, and then determine the absorbance with A = - log T.